Journal: The EMBO Journal

Article Title: NRAC controls CD36-mediated fatty acid uptake in adipocytes and lipid clearance in vivo

doi: 10.1038/s44318-025-00520-2

Figure Lengend Snippet: ( A ) Lipid tolerance test and area under the curve in mice fed high fat diet ( n = 7 wt, 7 ko, Age 16 weeks, 10 weeks on HFD), ( B ) serum triglyceride levels (17 weeks on HFD) ( n = 8 wt, 7 ko). ( C , D ) Body composition (13 weeks on HFD). Fatty acid uptake (FAU) assay in primary mature adipocytes from ( E ) PGF and ( F ) SCF ( n = 6 wt, 7 ko). Adipocytes were either treated with BSA or 100 µM oleate. ( G ) Immunostaining of differentiated adipocytes treated either with oleate (100 µM, 24 h) and ethanol (0.05%, 1 h) or with oleate (100 µM, 24 h) and CPZ (10 µM, 1 h) (scale bar 25 µm). ( H ) Immunostaining for NRAC and perlipin-1 (PLIN1) on PGF from chow diet (age 23 weeks) and HFD fed male mice (age 23 weeks, 17 weeks on HFD). Scale bar 25 µm. ( I ) Immunoprecipitation of Ubiquitin-HA. HEK293T cells were transfected with either NRAC or with NRAC + Ubiquitin-HA (Ub-HA) plasmids and treated with with BSA + MG132 (50 µM) or Oleate (100 µM) + MG132 (50 µM) for 1 h. Data are shown as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 by Student’s t test or one or two-way ANOVA. .

Article Snippet: The mice were fed either a standard chow diet (Altromin 1314, Lage Germany) or a 58% high-fat diet (HFD) (Research Diets D12331 , New Brunswick (NJ), USA).

Techniques: Immunostaining, Immunoprecipitation, Ubiquitin Proteomics, Transfection